Wastewater treatment using bamboos in constructed wetlands: experiences and future perspectives.

Wastewater treatment using constructed wetlands (CWs) based on natural wetlands constitute a viable alternative with excellent cost and benefit, presenting themselves as efficient technologies in the secondary and tertiary treatment of wastewaters with low implementation, operation, and maintenance costs. The present study aims to evaluate the use of bamboo species, as an alternative to macrophytes, frequently used in CWs, through bibliometric analysis, besides to a review based on case studies. The maps generated by the VOSviewer software and by the analyses of the Web of Science and Scopus databases allowed for a review of typical concepts of CWs, in addition to revealing potential benefits of using bamboos in CWs, such as their hyperaccumulation capacity and bioproduct generation. Other promising aspects were identified, for example the use of bamboo charcoal as a substrate used in subsurface wetlands and the application of ornamental bamboo species for landscape improvements, among other observations. The efficiencies found in six case studies showed values between 89-99.7%, 47.6-99.7%, 58.3-99.9%, and 85.5-99.8% for BOD5, COD, total nitrogen (TN), and total phosphorus (TP), respectively. Despite the promising results, the lack of studies using bamboos in CWs for the treatment of wastewaters limits an assertive statement about the use of this technology, requiring further research, focusing on the morphological functions of bamboos in this treatment with landscape integration and resources recovery.

Combined system for wastewater treatment: ozonization and coagulation via tannin-based agent for harvesting microalgae by dissolved air flotation.

This study evaluated the performance of urban wastewater treatment in pilot-plant by an integrated system consisting of anaerobic reactor, microalgae cultivation, Venturi tube ozone recirculation, coagulation/flocculation with tannin-based agent natural coagulant, and dissolved air flotation (DAF). Ozone concentrations (without ozone, 0.13 and 0.25 mg O3/mg of biomass) and tannin dosages (65, 85 and 105 mg/L) were evaluated regarding microalgae separation and their influences on wastewater treatment performance. During the experiments, it was verified that the treatment efficiency increased when ozone was applied and with higher tannin dosages. The best results were found with 0.13 mg O3/mg of biomass and 105 mg/L of tannin, obtaining excellent removal of turbidity removal (99.4%), apparent colour at 420 nm (94.5%), TN (83.2%), N-NH3 (100%), TOC (86.8%), BOD5 (86.5%) and COD (100%), 47.6% reduction in electric conductivity, 46.1% in TDS, 66.4% TP removal for the integrated system and 84% microalgae biomass recovery were obtained. Our results showed that the system proved to be a viable alternative for the treatment of urban wastewater and the recovery of microalgae through the insertion of ozone via Venturi tube combined with tannin-based agent.

Hybrid constructed wetlands integrated with microbial fuel cells and reactive bed filter for wastewater treatment and bioelectricity generation.

The present study aimed to develop a pilot-scale integrated system composed of anaerobic biofilter (AF), a floating treatment wetland (FTW) unit, and a vertical flow constructed wetland coupled with a microbial fuel cell (CW-MFC) and a reactive bed filter (RBF) for simultaneously decentralized urban wastewater treatment and bioelectricity generation. The first treatment stage (AF) had 1450 L and two compartments: a settler and a second one filled with plastic conduits. The two CWs (1000 L each) were vegetated with mixed plant species, the first supported in a buoyant expanded polyethylene foam and the second (CW-MFC) filled with pebbles and gravel, whereas the RBF unit was filled with P adsorbent material (light expanded clay aggregate, or LECA) and sand. In the CW-MFC units, 4 pairs of electrode chambers were placed in different spacing. First, both cathode and anode electrodes were composed of graphite sticks and monitored as open circuit. Later, the cathode electrodes were replaced by granular activated carbon (GAC) and monitored as open and closed circuits. The combined system efficiently reduced COD (> 64.65%), BOD5 (81.95%), N-NH3 (93.17%), TP (86.93%), turbidity (94.3%), and total coliforms (removal of three log units). Concerning bioenergy, highest voltage values were obtained with GAC electrodes, reaching up to 557 mV (open circuit) and considerably lower voltage outputs with closed circuit (23.1 mV). Maximum power densities were obtained with 20 cm (0.325 mW/m2) and 30 cm (0.251 mW/m2). Besides the electrode superficial areas, the HRT and the water level may have influenced the voltage values, impacting DO and COD concentrations in the wastewater.

Floating treatment wetlands integrated with microbial fuel cell for the treatment of urban wastewaters and bioenergy generation.

The objective of the present study was to develop a combined system composed of anaerobic biofilter (AF) and floating treatment wetlands (FTW) coupled with microbial fuel cells (MFC) in the buoyant support for treating wastewater from a university campus and generate bioelectricity. The raw wastewater was pumped to a 1450 L tank, operated in batch flow and filled with plastic conduits. The second treatment stage was composed of a 1000 L FTW box with a 200 L plastic drum inside (acting as settler in the entrance) and vegetated with mixed ornamental plants species floating in a polyurethane support fed once a week with 700 L of wastewater. In the plant roots, graphite rods were placed to act as cathodes, while on the bottom of the box 40 graphite sticks inside a plastic hose with a stainless-steel cable acting as the anode chamber. Open circuit voltages were daily measured for 6 weeks, and later as closed circuit with the connection of 1000 Ω resistors. Plant harvestings were conducted, in which biomass production and plant uptake from each of the species were measured. On average, system was efficient in reducing BOD5 (55.1%), COD (71.4%), turbidity (90.9%) and total coliforms (99.9%), but presented low efficiencies regarding total N (8.4%) and total P (11.4%). Concerning bioenergy generation, voltage peaks and maximum power density were observed on the feeding day, reaching 225 mV and 0.93 mW/m2, respectively, and in general decaying over the 7 days. In addition, plant species with larger root development presented higher voltage values than plants with the smaller root systems, possible because of oxygen release. Therefore, the combined system presented potential of treating wastewater and generating energy by integrating FTW and MFC, but further studies should investigate the FTW-MFC combination in order to improve its treatment performance and maximize energy generation.

Acute ecotoxicity and genotoxicity assessment of two wastewater treatment units.

Water contamination by discharge of untreated or poorly treated wastewater into water bodies is a current issue that may cause harm to humans. Water quality assessment targets general parameters, which often misleads to underestimation of their implication in the environment. Acute and genotoxicity assays using Daphnia magna is a reliable tool for testing deleterious effects of wastewater exposure. This paper aimed at evaluating acute ecotoxicity as well as genotoxicity of a biological treatment system composed by an anaerobic bioreactor (AR), algal turf scrubber (ATS), followed by two downflow constructed wetlands (CW). The university's wastewater treatment plant (WWTP) composed by an equalization tank (ET), an upflow anaerobic sludge blanket (UASB), followed by an aerobic bioreactor (AB) was also assessed for acute and genotoxicity. Our results showed the acute ecotoxicity ranged from moderately to extremely toxic, and from nontoxic to moderately toxic for ET and AB. For AR, most samples were moderately toxic. The outflow from ATS and CW's tanks completely eliminated acute toxicity and genotoxicity evidencing that the proposed system combining ATS and vertical CWs is suitable for treating sanitary wastewater.

Combined system for the treatment and reuse of urban wastewater: the efficiency of anaerobic reactors + hybrid constructed wetlands + ozonation.

The research developed a combined system in batch flow and in pilot scale for the treatment and reuse of urban effluents. The system was fed raw effluent from a university campus in Brazil and composed of four anaerobic reactors, three constructed wetlands (CWs) and an ozonation unit. The three sequential hybrid constructed wetlands were composed of a floating treatment wetland, an aerobic-anoxic baffled constructed wetland (CW) and a saturated vertifcal flow CW. Later, during the last trimester, weekly samples of the treated effluent were ozonated by bubbling with an application rate of 240 mg.h-1 O3. The system presented high removal rates efficiencies in terms of carbonaceous organic matter (78.9%), nitrogen (91.0%), color (96.7%) and turbidity (99.1%). In addition, it worked well for disinfection and acute ecotoxicity, but P was only efficiently (75%) removed in the first 8 months, with removing efficiency declining after this period. Ozonation provided significant color removal and an increased pH. The combination of floating, alternated upflow and downflow and saturated vertical flows improved the treatment of wastewater. This was due to the presence of both aerobic and anaerobic zones, as well as the filter substrate, through an integrated system with simple construction and operation and increased lifespan.

Algae turf scrubber and vertical constructed wetlands combined system for decentralized secondary wastewater treatment.

Water shortage is a current problem faced by many regions. The deterioration of water bodies driven by the directly discard of untreated wastewater worsens the water shortage and implies in more costly treatments to meet local standards for water quality. In rural areas, the problem is even worse, once conventional centralized treatment plants do not encompass them. Decentralized treatment systems must present low-cost, local availability, standards-meeting efficiency, and simplified operation. The present study examines the combined use of algae turf scrubber and down-flow vertical constructed wetlands for a University's sanitary wastewater treatment. After a hydraulic detention time of 21 days, the unit was able to reach 49%, 48%, 98%, 82%, 99.2%, 70.1%, 44%, 83%, 72%, 86%, 69%, 95%, and 99.9% for conductivity, total soluble solids, turbidity, apparent color, N-NH3, total nitrogen, P-soluble, total carbon, chemical oxygen demand, inorganic carbon, TOC, Escherichia coli, and total coliforms. In accord to the Brazilian standard ABNT 13969/97, the treated effluent is eligible for reuse in floor and sidewalks washing, garden irrigation, and landscaping purposes.